Generator

Part:BBa_K558001:Design

Designed by: Jovanna Casas   Group: iGEM11_Nevada   (2011-08-25)

s70/PDC/ADH Ethanol Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1066
    Illegal AgeI site found at 1213
    Illegal AgeI site found at 1591
    Illegal AgeI site found at 2362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 100
    Illegal BsaI site found at 2713
    Illegal SapI site found at 514
    Illegal SapI site found at 1177


Design Notes

A stuffer region between the PDC and ADH coding regions includes restriction digest sites for NotI and XhoI.

Source

The sequence for these genes is based on Zymomonas mobilis, a microorganism that naturally produces ethanol.

References

Conway T, Sewell G W, Osman Y A, Ingram L O. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J Bacteriol. 1987;169:2591–2597.

Ingram, L.O., Conway, T., Clark, D.P., Sewell, G.W. and Preston, J.F. (1987, Oct) Genetic Engineering of Ethanol Production in Escherichia Coli. Applied and Environmental Microbiology: 2420-2425.